Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron. It works by reducing insoluble ferric iron (Fe³⁺) to ferrous iron (Fe²⁺) and binding with it to form a highly visible purple complex.

Thioglycolic acid is used for the reduction of iron(III) to iron(II), and for pH adjustment. Thus only one reagent is required for analysis.

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TPTZ method is most common method for low range Iron determination from the sample.

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it can be used to clean laboratory glassware, particularly of otherwise to clean insoluble organic residues.

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The copper sulfate test is used to detect the presence of metallic iron and iron oxide on the surface of materials.

Question – What solution strength is needed to copper sulfate solution can be used to determine if a metal is stainless steel or just steel ? 
Answer    – Copper sulfate (CuSO4*5H2O) + H2SO4 (anywhere from 1% to 50% is specified in various test methods).

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