THIOGLYCOLATE INDICATOR

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron.

SKU: RXSOL-62-6047-111 Category:

Description

Thioglycolate (thioglycolic acid) is used in water testing as a powerful reducing and complexing agent to measure both dissolved and total iron. It works by reducing insoluble ferric iron (Fe³⁺) to ferrous iron (Fe²⁺) and binding with it to form a highly visible purple complex.

Thioglycolic acid is used for the reduction of iron(III) to iron(II), and for pH adjustment. Thus only one reagent is required for analysis.






Product Details

Application

How the Thioglycolate Method Works

 

  • Reduction: Thioglycolic acid reduces oxidized ferric iron (Fe³⁺) into soluble ferrous iron (Fe²⁺).
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  • Complexation: The ferrous iron reacts with the thioglycolate to form a purple or pink-colored ferrous thioglycolate complex.
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  • Measurement: The intensity of the resulting color is directly proportional to the total iron concentration in the water sample
  • Typical Testing Procedure
    1. Color Comparison: After a brief waiting period (usually 3 to 5 minutes), the color of the sample is compared against a standard color chart or measured photometrically to determine the iron level in mg/L (or ppm).
    Advantages and Limitations
    • Interferences: The formulation used in many test kits is designed to minimize interferences from other heavy metals that might be present in the water sample

    Dosage & Procedure

    Typical Testing Procedure

     

  • Sample Preparation: A measured water sample is placed into a test tube or flask.
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  • Reagent Addition: Citric acid is often added first to prevent the precipitation of iron, followed by the thioglycolate indicator.
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  • pH Adjustment: Ammonia is added to make the solution alkaline. This step is crucial for the colored complex to form.

    Color Comparison: After a brief waiting period (usually 3 to 5 minutes), the color of the sample is compared against a standard color chart or measured photometrically to determine the iron level in mg/L (or ppm)
  •  

    Notes & Precautions

    A thioglycollate indicator (typically resazurin) is a redox dye used to visually map oxygen levels in bacterial culture tubes. 
    In its oxidized state, it turns pink when exposed to oxygen; in its reduced, oxygen-free state (typically at the bottom), it remains colorless.
     
    Resazurin Indicator Characteristics
    Pink Zone: Indicates the presence of oxygen (aerobic zone) at the top of the tube.
    Colorless Zone: Indicates the absence of oxygen (anaerobic zone) deeper in the tube.
    Reversibility: Resazurin is highly sensitive; if the broth is shaken, it re-oxidizes and the top portion will turn pink until the thioglycolic acid reduces the oxygen again

    Generic / Chemical Name

    Iron Test Kit, colorimetric

    Supply Locations

    Iron Test Kit manufacturer supplier distributor in Mumbai, Kandla, Kolkata, Vizag, Chennai, India, Fujairah, Dubai UAE, Muscat Oman, Kenya Africa. Get the best quality of Iron Test Kit at a competitive price from us. We have ready stock of Iron Test Kit in India, UAE Gulf, Oman, Kenya Africa. Contact us for bulk as well as small orders.

    Safety Data (MSDS)

    Product Name
     

    Iron Test Kit

    Product Type
     

    RXSOL-62-6047-001

    Company Details:
    RX MARINE INTERNATIONAL
    105, A wing , BSEL , TECH PARK.
    VASHI ,NEW BOMBAY 400703 INDIA

    Phone +91 22 27815540 / 41 / 42
    Fax +91 22 2781 1318 :::AOH :0091 9322594669
    Email mail@rxmarine.com

    Ingredients Conc. (%) CAS # EC #
    Hydroxylamine hydrochloride <1 5470‐11‐1 226‐798‐2
    2,4,6‐Tri (2‐pyridyl)‐1,3,5‐ triazine <1 3682‐35‐7 222‐965‐9
    Hydrochloric acid <0.5 7647‐01‐0 231‐595‐7
    Water Q.S. 732‐18‐5 231‐791‐2

    WHMIS            Classification
      E (Corrosive)
    D‐2A (Very Toxic)

    Eye contact                                                              Immediately flush with copious quantities of water for a minimum of 15 minutes holding lids apart to ensure flushing of the entire surface. Seek immediate medical attention.
    Skin contact Remove the contaminated clothes and flush with plenty of water and soap. Seek medical attention is irritation symptoms persist.
    Inhalation If inhaled, remove patient to fresh air. If symptom persists, obtain medical attention.
    Ingestion Have conscious person drink several glasses of water or milk. DO NOT induce vomiting. Obtain medical attention immediately.

    Suitable extinguishing media:                                                         Use extinguishing media appropriate to surrounding fire conditions.
    Specific hazards arising from the chemical: Hydrogen chloride.
    Special protective actions for fire‐fighters: Wear adequate personal protection to prevent contact with material or it combustion products. Self‐contained breathing apparatus with a full‐face piece operated in a pressure demand or other positive pressure mode.

    Person‐related safety precautions:                                             Rubber or plastic gloves. Full face‐shield or chemical splash goggles or face mask. Protective clothing, apron, rubber boots.
    Measures for environmental protection: DO NOT empty into drain.
    Measures for cleaning/collecting: Absorb spill with inert material such as clays, sand, earth, or vermiculate and place in closed container for disposal. Clean the spill area after product complete removal.

                                                                                Store away from bases, reducing and oxidizing agent, sodium, fluoride, calcium carbide. Do not store with incompatible materials, even in case of spill. Store in cool, well‐ventilated area. Keep containers closed and in upper position.

    Protective clothing                                                        Rubber or plastic gloves. Full face‐shield or chemical splash goggles or face mask. Protective clothing, apron, rubber boots.
    Engineering controls General ventilation is recommended.

    Appearance (physical state, color, etc) / Odor Liquid. Odourless and Colourless.
    Odor threshold Not available
    pH < 2
    Melting point / Freezing point 0 °C
    Initial boiling point and boiling range Not available
    Flash point Not available
    Evaporation rate Not available
    Flammability (solid, gas) Not available
    Upper / Lower flammability Not available
    Vapor pressure Not available
    Vapor density Not available
    Relative density Not available
    Solubility Miscible in water
    Partition coefficient: n‐octanol/water Not available
    Auto‐ignition temperature Not available
    Decomposition temperature Not available
    Viscosity Not available

    Instability conditions                                                This product is stable under normal conditions.
    Conditions to avoid Avoid high temperatures and contamination.
    Materials to avoid Alkalis, oxidizing and reducing agents, sodium, fluoride, calcium carbide, metals.
    Hazardous decomposition products Toxic gas of hydrogen chloride.
    Hazardous polymerization Does not polymerize

    Route of exposure                                                     Conclusion / Remarks
    Skin corrosion/irritation Causes burns if he exposed area is not washed immediately.
    Serious eye damage/irritation Cause severe burns. May cause permanent damage.
    Respiratory or skin sensitization If inhaled, may cause nose and throat irritation.
    Ingestion If ingested burning of the mouth, pharynx and abdomen.
    Hydroxylamine hydrochloride
    Oral (LD50): Acute 400 mg/kg (mouse)
    Hydrochloric Acid
    Oral (LD50): Acute 900 mg/kg (rabbit)
    LC50: 3,124 ppm (rat ‐1hr.)
    Germ cell mutagenicity Laboratory experiments have shown mutagenic effect of Hydroxylamine hydrochloride.
    Carcinogenicity Not available
    Reproductive toxicity Not available
    STOT‐single exposure Not available
    STOT‐repeated exposure Not available
    Aspiration hazard Not available
    Acute toxicity Corrosive! Harmful if ingested or inhaled. Causes severe burn
    Chronic Overexposure Laboratory experiments have shown mutagenic effect of Hydroxylamine hydrochloride. Target organ: blood.

    Ecotoxicity Not available
    Mobility Not available
    Persistence and Degradability Not available
    Bioaccumulation potential Not available

    Disposal recommendations                                      Dispose of in an approved landfill in accordance with federal, provincial and local regulations.
    Regulatory disposal information Not available

    Reagent
    TDG CLASS 8. PIN UN1789, HYDROCHLORIC ACID PG:III
    Kit
    TDG CLASS 9. PIN UN3316

     

    Not available

    The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Rx Marine International be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Rx Marine International has been advised of the possibility of such damages.

    DISCLAIMER OF LIABILITY :The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling, storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable.

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